a-Actinin and Tropomyosin Interactions with a Hybrid Complex of Erythrocyte
نویسندگان
چکیده
a-Actinin isolated from dog muscle was used to incite antibodies in rabbits. Antibodies, purified by affinity chromatography on CNBr-Sepharose coupled with a-actinin and then ferritin-labeled were found to localize on the Z disc of muscle sarcomeres. Molecules of a-actinin as an adsorbed monolayer on the surface of polystyrene Lytron particles could bind muscle. actin and tropomyosin from solution. Both the ATPase activity and superprecipitation of an erythrocyte . actin and muscle. myosin hybrid actomyosin complex were altered by a-actinin, while tropomyosin diminished these a-actinin effects. The binding properties of a-actinin are consistent with those of an anchoring protein for microfilaments in nonmuscle cells.
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